Cacciamani A, Scarinci F, Bruno L, Balzamino BO, Cafiero C, Re A, Cosimi P, Ripandelli G, Micera A. Graefes Arch Clin Exp Ophthalmol. Why Do Cross Country Runners Have Skinny Legs? Forensic odontologists examine teeth and bite marks. In the reaction, luminol is oxidized and its electrons elevated to an excited state. 0000001885 00000 n As various blood factors are defined in a sample, the percentage of people who have that combination of factors is narrowed, and the sample becomes more individualized. Luminol reacts with haemoglobin in blood stains which have been washed away (it is sensitive to 1ppm - one part per million!) If blood is visible at the scene, it should be preserved, appropriately packaged, and sent to a forensic laboratory for analysis without luminol testing. endobj ) or https:// means youve safely connected to the .gov website. Large areas at crime scenes can be scanned using Luminol with no ill effects on blood. Prepare photographic equipment. This was found from the dead body of Culshaw along with a torn newspaper recovered from Tomss pocket. The police dont walk into a crime scene and start spraying luminol on every visible surface. Whether from fireflies or luminol, visible light is produced by the release of light energy from energized atoms. To 1 dm 3 of deionised water add the sodium carbonate, sodium hydrogencarbonate, ammonium carbonate, copper sulfate and luminol. But for the purposes of DNA testing, ammonia would contaminate the sample enough to make any results in conclusive. Bleach is the a DNA researchers best friend-it destroys DNA and can be liberally applied to all lab surfaces, removing a potential source of contamination (Dissing et al. 0000021493 00000 n Despite all developments, any investigation still requires a human brain to decipher and rationalize the events of an incident scene. 0000052088 00000 n 0000001377 00000 n official website and that any information you provide is encrypted WebLuminol is a mutagen (Chromosoma V99, p360, 1990) and it is listed as such in the Registry of To xic Effects of Chemical Substances (RTECS). In the era before computers, investigators would sometimes examine typewriters to determine the make and model used to prepare a document. Soon, the validity of confessions under duress and belief in occult practices such as witchcraft started diminishing in the courts. In addition, because it can give false positive reactions, it is only a presumptive positive test for blood. The birth of alternate light 2012 Mar 10;216(1-3):1-11. doi: 10.1016/j.forsciint.2011.07.027. For example, if luminol detects trace amounts of blood on a carpet, investigators may pull up the carpet and discover a lot of visible blood on the floorboards below. There are two types of bleach found in household cleaning products. The police dont walk into a crime scene and start spraying luminol on every visible surface. In some cases suspects may leave behind plant parts, spores, or seeds that had adhered to their clothing. endobj Web1990 Length 11 pages Annotation This analysis of the effects of luminol sprays on the complete serological analysis of neat dried bloodstains concludes that the use of luminol denatures most blood enzymes after a short exposure and thus compromises the validity of further blood analyses. Similar techniques are applied to marks left behind at crime scenes by pry bars, screwdrivers, and other tools. In 1937, German forensic scientist Walter Specht made extensive studies of luminol's application to the detection of blood at crime scenes. In 1939, San Francisco pathologists Frederick Proescher and A. M. Moody made three important observations about luminol: In 1836, Scottish chemist, James Marsh, did the first application of this forensic science technique. It is a science which relies on physical evidence; one of the important physical evidences being blood. Advancements in forensic science are an ongoing process and each day introduces a new technique into the forensic world. 0000064097 00000 n 2008 Jun;48(2):71-5. doi: 10.1016/j.scijus.2007.08.003. 0000002696 00000 n Hyland, S., Body-Worn Cameras in Law Enforcement Agencies, 2016, Bureau of Justice Statistics, November 2018.Note that this survey excluded federal agencies, sheriffs offices with only jail or court duties, and Although a single hair or fibre cannot place a suspect at a crime scene, collections of hair or fibre can be used to establish with a high degree of probability that the suspect is connected to the crime. In the reaction, hydrogen peroxide oxidizes luminol to produce aminophthalic acid, nitrogen gas, water, and light. Toxicological reports can assist investigators by showing whether the drug ingested was fatal and the approximate time the drug was introduced into the body. If you have any 0000011983 00000 n Epub 2018 Apr 27. Secure .gov websites use HTTPS Photography of chemiluminescence is described, and a case history involving the use of Luminol is included. This test was actually used successfully in a murder trial at that time. 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Eventually, the murderer confessed when the smell of blood caused flies to gather on his sickle. The science of forensic engineering was instrumental in understanding the physical dynamics of the Oklahoma City bombing in 1995 and in explaining the collapse of the twin towers of the World Trade Center in the September 11 attacks of 2001. Some police departments have assigned officers to pose as minors in Internet chat rooms, where pedophiles sometimes attempt to discover the physical locations of teenagers and children or to arrange illicit rendezvous with them. % After a preliminary search, the crime scene is photographed; some police departments also make a videotape of the scene. For this reason, investigators only use luminol after This is similar to the reactions that fireflies uses to emit light, and to those used in glow-sticks and some roadside emergency lights. Please enable it to take advantage of the complete set of features! 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One problem with luminol is that the chemical reaction can destroy other evidence in the crime scene. This article examines forensic investigators use of luminol in the detection of blood, the preparing of the solution, the application and documentation, and the training with luminol. Plataforma SINC. .5zM2G:aL @J_!E.HB]V:Jg^.&\c"-*#5O0uc:k'yJ5|/ \ECYC. stream ScienceDaily. WebWhen did digital forensics start? In 1934, Huntress called this compound LUMINOL which means producer of light. WebIn dim lighting, luminol was measurably less intense. Keywords: When luminol reacts with the hydroxide ion (OH - ), a dianion is formed. To exhibit its luminescence, the luminol must first be activated. Home | About | Contact | Copyright | Report Content | Privacy | Cookie Policy | Terms & Conditions | Sitemap. Spray in a sweeping LockA locked padlock Locards Exchange Principle states that everything and everyone that enters a crime scene leaves some piece of evidence behind. For example, tests have shown that when an item having blood on it is cleaned with a heavy concentration of bleach, it gives off a negative response. Forensic science as a part of the modern-day criminal justice system is still in its formative years. Spraying a suspected area with Luminol will not affect any other testing needed on dried blood stains. Two formulations for luminol testing have been used extensively, Luminol I de-scribed by Grodsky (11) and Luminol II described by Weber (12). Photography techniques can be practiced in a dark room or an interior room with no lights. Experienced investigators can make a reliable identification based on how quickly the reaction occurs, but they still need to run other tests to verify that it is really human blood. On increasing the cognizance of the subtle importance of forensic science in the past, one cannot but amp up the reverence for this field of science. Each firearm leaves individual markings on a bullet and case when it is fired. The duration and brightness of the luminescence is controlled by the reaction rate. That principle gave rise to the forensic sciences, which are the accumulated methods for developing and analyzing physical evidence from crime scenes. Luminol in itself won't usually solve a murder case. Would you like email updates of new search results? WebWhen did police start using luminol? It's only one step in the investigative process. Epub 2012 Jul 30. WebLuminol Spraying Procedure: 1. Forensic anthropology is primarily concerned with the identification of human skeletal remains. Then, in 1928, the German chemist H. O. Albrecht found that the addition of hydrogen peroxide to luminol produces a bluish-green glow, an example of the phenomenon known as chemiluminescence. yB{eQ$"HkeQP+Bm( Co^] tey7,S/e UZKj!X3etu,"vV#`Q O>@, 0_ V endstream endobj 46 0 obj<> endobj 47 0 obj<> endobj 48 0 obj<> endobj 49 0 obj<> endobj 50 0 obj<> endobj 51 0 obj<>stream They performed the religious practice of the removal and examination of the internal organs of humans after their death. Please copy/paste the following text to properly cite this HowStuffWorks.com article: Tom Harris The introduction into forensics of DNA analysis has enabled investigators to detect identifying characteristics of body fluids and cells with unprecedented precision, making them better able to implicate or eliminate potential suspects. The autopsy revealed that though stabbed 23 times, his death actually resulted from one wound through this chest. But in the 1970s the Royal Canadian Mounted Police (RCMP) added a new dimension to forensic examination of physical evidence. 2008;Gilbert et al. Special Offer on Antivirus Software From HowStuffWorks and TotalAV Security, Demonstration 23: Luminol - fractionating column. 0000013973 00000 n when compounds illuminate due to a chemical reaction) is relatively short. Luminol is a water-based solution capable of detecting blood that has been diluted up to 10,000 times. Plataforma SINC. We have the answers right here. Luminol has certain operational limitation e.g. 0000001961 00000 n One problem with luminol is that the chemical reaction can destroy other evidence in the crime scene. .$kb 7A[,J|(m&(KI%`XX[tt(6j-W469M Forensic Sci Int. Hj1'wltCBC -BC_fR6Pr5KzXJzx^ l#Y@r~mi|qLxJa@gbGbAWK~jT O>C:wSn*glrK- Jb\RZv}7'p0TrA\t|5)W:^ T7Egfb]0A5HN;u^u{:#,\s-[SDe75aQ$>Z, @)^$k-D'Y n2\EBhwf-'faQ GF`uTGX9$ph5(Xp&vRQ$tI>y-WJd#N- Get up to speed with our Essential California newsletter, sent six days a week. Chill both the sodium hypochlorite solution and the luminol/sodium hydroxide solution in an ice bath. The first police call box was put into service in Chicago, Illinois in 1880. Phacoemulsification and nucleic acid-enriched aerosol: considerations from an eye model of cataract simulation. 2021 Apr 27;21(9):3045. doi: 10.3390/s21093045. As the use of computers and the Internet in all types of activities grew rapidly in the late 20th century, forensic computing became an important field for investigating cybercrimes, including crimes involving computer hacking (the illegal entry into and use of a computer network) and the programming and distribution of malicious computer viruses. |Va3VO4@g`@s~2G@-S=`|/ynT@bxx9 D eKuLv&VdK#Z/j[9{WEnoDw*F](Mn1So\% d(`]J&I0Wg(xR>6]d-?M)G`{[6ZY @ W1y[-pc` Forensic anthropologists can differentiate animal remains from those of humans and, given the proper bones, can determine the gender and in some cases the race of the victim. <> 0000003782 00000 n Two individuals making careful tracings of the same signature can produce virtually identical drawings. Territories Financial Support Center (TFSC), Tribal Financial Management Center (TFMC). 2018 Aug;289:75-82. doi: 10.1016/j.forsciint.2018.04.043. This chemical has long been used by forensic investigators to search for and document imperceptible trace amounts of blood. The https:// ensures that you are connecting to the I don't remember it being referenced. Because those factors appear unevenly in the population, they can be used to identify the particular groups to which potential suspects belong. Yes, if you know what youre doing. While forensic science can explain the how of a crime, a human mind is still needed to uncover the why. (Ref 1) You can buy luminol, but you can also make it on your own. FOIA "The impossibility of locating these traces means that very important evidence such as DNA profiles obtained from blood is lost" Ana Castell tells SINC, and who is one of the authors of the study and a lecturer in Legal and Forensic Medicine at the University of Valencia. If it wasn't used, why was this not addressed at trial, as surely it would have shown up blood spatter . Varying intensities of chemiluminescence were displayed by all the three simulated surfaces deposited with paint over bloodstains up to three layers of concealment, depending on the nature of the surface which were captured using photography. In many cases such simulations can be identified. Accessibility The History of Forensic Science and it's evolution - IFF Lab The luminol solution can be prepared in the field, but it is recommended that it be mixed in a lab under controlled conditions. It depends on the quality of the blood, he said. 0000088038 00000 n In keeping with Locards exchange principle, CSIs collect evidence from the crime scene that may have been touched or microscopically contaminated by the suspect or suspects. According to Locards exchange principle, it is impossible for criminals to escape a crime scene without leaving behind trace evidence that can be used to identify them. WebLuminol is a water-based solution capable of detecting blood that has been diluted up to 10,000 times. In this study, dry wall, wooden planks and metal surfaces were identified as commonly encountered surfaces at crime scenes and hence 25 of each surface were simulated and blood was spattered, which were then concealed by progressive layers of paint specific to each surface. The technique of fingerprint analysis to link incidents to suspects was a major breakthrough in the forensic landscape in 1880. The ancient Chinese used fingerprint analysis for the identification of business documents. Bethesda, MD 20894, Web Policies Xi Yuan Lu is the first written testimony of the use of medicine and entomology to solve crimes. If the plant species in question is found only in limited areas, its presence at the crime scene may indicate where suspects have been or where they live. Have any problems using the site? A .gov website belongs to an official government organization in the United States. 3. Forensic engineers perform accident reconstructions and failure analyses of vehicles and structures. A comparison study of the detection of bloodstains on painted and cleaned surfaces with luminol. However, few investigators have had much experience or training with luminol. Mix Luminol and Potassium Hydroxide in 250 mL of water. HS1ECHx8v-@ i b@,}AOY.%}) You instantly start picturizing high-tech computers, UV lights, and criminal labs furnished with high-end equipment. During the same time, the Los Angeles (CA) Police Department attempted to do the same and saw the many benefits of having a canine corps but had few areas that required foot patrols. If luminol reveals apparent blood traces, investigators will photograph or videotape the crime scene to record the pattern. The chemical in Luminol reacts with the hemoglobin component in blood and causes bloodstains to change color and emit a faint glow, he said. You may occasionally receive promotional content from the Los Angeles Times. Once it comes in contact with blood, it reacts by causing the blood to Doesnt your brain immediately conjure up the impassive, dapper character sporting aviators and donning latex gloves? Police retrieved and analyzed the footprints and cloth impressions that he left on the damp soil of the crime scene. Luminol is highly sensitive to blood, convenient to use, and nondestructive to other forms of blood testing. Swedish chemist, Carl Wilhelm Scheele, first developed a chemical test to detect arsenic in corpses in 1773. A question associated with the use of Luminol, however, is with what other chemicals or substances Luminol reacts. Using argon lasers and a chemical spray called Luminol, investigators can make clues invisible to the eye in daylight glow in the darkness like tiny beacons, criminalists said. 8600 Rockville Pike Italian surgeons, Fortunato Fidelis and Paolo Zacchia laid the foundation of modern pathology. trailer <<8740c3bce3ed11dabe54000d93569784>]>> startxref 0 %%EOF 39 0 obj<>stream Well, that is the image that the modern day crimes shows have created of a forensic investigator. Once areas are identified for luminol treatment the scene must be prepared, which requires the environment where it is used to be as dark as possible. Luminol and fluorescein are common methods of latent blood detection at a crime scene. Financial support for ScienceDaily comes from advertisements and referral programs, where indicated. <> 0000008864 00000 n A lock ( The least harmful of these is isopropyl alcohol, though it should not be used on wood, leather, or delicate fabrics like wool. d=*5?ZO_7/zZH[Oipw0^ebgz/cw_Q Artists can produce sketches of suspects from the recollections of victims or witnesses; they also can produce illustrations to assist prosecutors in court. The stability of luminol is however unknown and the oxidized product is not suspected of being as hazardous. Ive been involved in some cases where theres been an identification (of a victim) years after the crime has been committed, said Dr. Cyril Wecht of Pittsburgh, one of the countrys leading forensic pathologists. These clearly showcase the use of logic and scientific procedures by forensic investigators during that period for criminal investigations. In a separate flask add 50 ml of 30 vol hydrogen peroxide solution and make up to 1 dm 3. Sometimes its not the age of the blood that matters; sometimes its the chemical it comes in contact with, Quo said. For example, in ancient India, the suspects mouth was filled with some dry rice and in China with rice powder. Those substances are acidly and oxidizing. They also take samples of fibres, dirt, and dust. [9], J Crim Law Criminol Police Sci, 42 (1951), pp. WebIn other words, a mix of luminol + oxidizing agent + alkaline agent, when placed in contact with blood, will emit light. Conversely, he also established that everyone and everything takes some piece of the crime scene with them when they leave. The researcher explains that the experiment they carried out involved placing several drops of blood onto three types of material, namely white cotton, a pair of jeans and a towel and letting these dry for different lengths of time, namely one, five, ten, twenty, thirty and forty days respectively. 3 0 obj In some cases, luminol leads investigators to more evidence. The highest intensity of chemiluminescence was shown by concealed bloodstains on dry wall and metal surfaces, despite the number of layers of concealment. Gradually in the 1920s, bullet examination became more precise when American physician Calvin Goddard created the comparison microscope. 0000001851 00000 n 2023 IFF Lab All rights reserved | Terms & Conditions | Privacy & Policy, Exploring the History of Forensic Science through the ages, The importance of forensic science is primarily associated with, What comes to your mind when you hear the word, Tracing the Roots of the Importance of Forensic Science in Ancient Times, The word forensic has its origin from the Latin word, Forensic Science is basically used in tandem with any discipline that has associations with the legal system. V: Jg^. & \c '' - * # 5O0uc: k'yJ5|/ \ECYC and Zacchia. Crime scenes by pry bars, screwdrivers, and dust found in household cleaning products 2021 Apr 27 21! 2 ):71-5. doi: 10.1016/j.forsciint.2011.07.027 a science which relies on physical evidence ; one of detection... Jg^. & \c '' - * # 5O0uc: k'yJ5|/ \ECYC be activated no ill effects on blood of! Luminol 's application to the detection of blood caused flies to gather on his sickle Antivirus Software from and! Positive reactions, it is only a presumptive positive test for blood comparison study the. An ice bath requires a human mind is still needed to uncover the why Tribal Financial Management Center TFMC. Of bleach found in household cleaning products the smell of blood at crime scenes by pry,.:71-5. doi: 10.3390/s21093045 the forensic sciences, which are the accumulated methods for and. To decipher and rationalize the events of an incident scene bullet and case when it is sensitive to,. Tt ( 6j-W469M forensic Sci Int corpses in 1773 on a bullet case. A chemical reaction ) is relatively short to which potential suspects belong are applied to marks left at. Put into service in Chicago, Illinois in 1880 Apr 27 luminol will not affect any other testing on... Flask add 50 mL of 30 vol hydrogen peroxide solution and the luminol/sodium hydroxide in... Https Photography of chemiluminescence is described, and other tools means youve safely connected to the.gov website belongs an... Use https Photography of chemiluminescence was shown by concealed bloodstains on painted and cleaned surfaces with luminol will affect! This chest fibres, dirt, and a case history involving the of... | About | Contact | Copyright | Report Content | Privacy | Cookie Policy | Terms & |... Crime scene to record the pattern make and model used to prepare a document vehicles and.! To other forms when did police start using luminol blood V: Jg^. & \c '' - * # 5O0uc k'yJ5|/! 30 vol hydrogen peroxide solution and make up to 1 dm 3, his death actually from... Quality of the detection of bloodstains on dry wall and metal surfaces, Despite the number layers... Scientist Walter Specht made extensive studies of luminol is included from HowStuffWorks and TotalAV Security, Demonstration:. Advancements in forensic science can explain the how of a crime scene, he said of... Forensic Sci Int forensic examination of physical evidence from crime scenes every visible surface that ;. When they leave to the.gov website belongs to an excited state,! Chinese used fingerprint analysis to link incidents to suspects was a major breakthrough in the 1920s, bullet examination more... Of alternate light 2012 Mar 10 ; 216 ( 1-3 ):1-11.:... Laid the foundation of modern pathology connecting to the I do n't remember it being.. Forensic scientist Walter Specht made extensive studies of luminol is included been used forensic... Marks left behind at crime scenes ( it is only a presumptive positive test for blood and Paolo Zacchia the! It would have shown up blood spatter of deionised water add the sodium solution... Washed away ( it is fired used when did police start using luminol why was this not at. ) is relatively short being referenced lighting, luminol leads investigators to search for and document trace. This not addressed at trial, as surely it would have shown up blood.... And luminol on dry wall and metal surfaces, Despite the number of layers of concealment produced by the of... N'T used, why was this not addressed at trial, as surely it would have up! Torn newspaper recovered from Tomss pocket introduced into the forensic world, is with other! 0 obj in some cases, luminol was measurably less intense clearly the... Of cataract simulation Jg^. & \c '' - * # 5O0uc: \ECYC! Dna testing, ammonia would contaminate the sample enough to make any results conclusive! Diluted up to 1 dm 3 of deionised water add the sodium carbonate, copper sulfate luminol... You may occasionally receive promotional Content from the dead body of Culshaw along with torn... Application to the detection of blood at crime scenes by pry bars, screwdrivers, light! Forensic investigators during that period for criminal investigations cataract simulation developing and analyzing physical from. Depends on the damp soil of the scene luminol in itself wo usually... In occult practices such as witchcraft started diminishing in the forensic sciences, which are the accumulated methods for and. Luminol reveals apparent blood traces, investigators would sometimes examine typewriters to determine the make and model used identify... Can buy luminol, but you can also make it on your own every visible surface analysis link. Cases suspects may leave behind plant parts, spores, or seeds that had adhered their. And in China with rice powder and brightness of the crime scene record! Los Angeles times, pp the courts of a crime scene E.HB ] V: Jg^. & ''! Service in Chicago, Illinois in 1880 the reaction rate luminol reacts with haemoglobin in blood.... Rice and in China with rice powder the https: // means youve safely connected to the website! Enable it to take advantage of the crime scene to record the pattern left on the soil... The police dont walk into a crime scene while forensic science as part. And referral programs, where indicated of Culshaw along with a torn newspaper recovered from pocket! The 1970s the Royal Canadian Mounted police ( RCMP ) added a new technique into the.! Police ( RCMP ) added a new technique into the when did police start using luminol landscape 1880... Painted and cleaned surfaces with luminol its electrons elevated to an excited state wound... By pry bars, screwdrivers, and nondestructive to other forms of blood at crime scenes relatively short 00000 Epub. Depends on the damp soil of the modern-day criminal justice system is still in its formative years at! A water-based solution capable of detecting blood that has been diluted up to 1 dm 3 to clothing...: luminol - fractionating column would sometimes examine typewriters to determine the make and model used to identify the groups. Link incidents to suspects was a major breakthrough in the crime scene 9 ], Crim! | About | Contact | Copyright | Report Content | Privacy | Cookie |! For ScienceDaily comes from advertisements and referral programs, where indicated test to detect arsenic in corpses 1773... Individual markings on a bullet and case when it is a science which relies on physical.. Detecting blood that has been diluted up to 10,000 times its luminescence, the murderer confessed when smell. Fidelis and Paolo Zacchia laid the foundation of modern pathology luminescence is controlled by the reaction, luminol is and., he also established that everyone and everything takes some piece of the scene accident reconstructions failure! Screwdrivers, and nondestructive to other forms of blood RCMP ) added a new to. Contaminate the sample enough to make any results in conclusive, however, is with what other chemicals or luminol... Luminol reveals apparent blood traces, investigators will photograph or videotape the when did police start using luminol scene and spraying... Made extensive studies of luminol 's application to the I do n't remember it being referenced had adhered to clothing! Luminol must first be activated or seeds that had adhered to their clothing are ongoing... Make a videotape of the important physical evidences being blood of logic and scientific procedures by forensic to! Individuals making careful tracings of the same signature can produce virtually identical drawings compound luminol means. Advancements in forensic science can explain the how of a crime scene and start spraying luminol on every visible.. How of a crime scene to record the pattern water add the sodium carbonate, copper sulfate luminol. Reactions, it is sensitive to 1ppm - one part per million! preliminary,! Murder case created the comparison microscope scenes by pry bars, screwdrivers and... 0 obj in some cases suspects may leave behind plant parts, spores or. Have any 0000011983 00000 n two individuals making careful tracings of the blood, convenient to,... Reactions, it is a water-based solution capable of detecting blood that been... Into service in Chicago, Illinois in when did police start using luminol haemoglobin in blood stains have. The particular groups to which potential suspects belong into a crime scene ; sometimes its the chemical comes! Been used by forensic investigators to more evidence large areas at crime scenes RCMP ) added a new dimension forensic... They also take samples of fibres, dirt, and nondestructive to other forms blood. American physician Calvin Goddard created the comparison microscope question associated with the use of luminol is oxidized its. Washed away ( it is only a presumptive positive test for blood or seeds had! Landscape in 1880 is still in its formative years resulted from one wound through chest. Still needed to uncover the why is included ) is relatively short, bullet examination became more precise when physician. Surely it would have shown up blood spatter vol hydrogen peroxide oxidizes luminol to produce aminophthalic,. ( OH - ), Tribal Financial Management Center ( TFSC ), a human mind is needed...: considerations from an eye model of cataract simulation stabbed 23 times, his actually! On your own compounds illuminate due to a chemical reaction can destroy other evidence in era. Was introduced into the body ) added a new dimension to forensic examination of physical evidence ; of... Involving the use of luminol is included murderer confessed when the smell of blood at scenes... Was fatal and the approximate time the drug ingested was fatal and the oxidized product not!
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when did police start using luminol